| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Integrative Study of Physiological Changes Associated with Bacterial Infection in Pacific Oyster Larvae
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| Autores: |
Genard, Bertrand
Miner, Philippe
Nicolas, Jean-louis
Moraga, Dario
Boudry, Pierre
Pernet, Fabrice
Tremblay, Rejean
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| Data: |
2013-05
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| Ano: |
2013
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| Resumo: |
Background: Bacterial infections are common in bivalve larvae and can lead to significant mortality, notably in hatcheries. Numerous studies have identified the pathogenic bacteria involved in such mortalities, but physiological changes associated with pathogen exposure at larval stage are still poorly understood. In the present study, we used an integrative approach including physiological, enzymatic, biochemical, and molecular analyses to investigate changes in energy metabolism, lipid remodelling, cellular stress, and immune status of Crassostrea gigas larvae subjected to experimental infection with the pathogenic bacteria Vibrio coralliilyticus. Findings: Our results showed that V. coralliilyticus exposure induced (1) limited but significant increase of larvae mortality compared with controls, (2) declined feeding activity, which resulted in energy status changes (i.e. reserve consumption, beta-oxidation, decline of metabolic rate), (3) fatty acid remodeling of polar lipids (changes in phosphatidylinositol and lysophosphatidylcholine composition,non-methylene-interrupted fatty acids accumulation, lower content of major C-20 polyunsaturated fatty acids as well as activation of desaturases, phospholipase and lipoxygenase), (4) activation of antioxidant defenses (catalase, superoxide dismutase, peroxiredoxin) and cytoprotective processes (heat shock protein 70, pernin), and (5) activation of the immune response (non-self recognition, NF-(kappa kappa) signaling pathway, haematopoiesis, eiconosoids and lysophosphatidyl acid synthesis, inhibitor of metalloproteinase and antimicrobial peptides). Conclusion: Overall, our results allowed us to propose an integrative view of changes induced by a bacterial infection in Pacific oyster larvae, opening new perspectives on the response of marine bivalve larvae to infections.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00144/25504/23662.pdf
DOI:10.1371/journal.pone.0064534
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| Editor: |
Public Library Science
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| Relação: |
http://archimer.ifremer.fr/doc/00144/25504/
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| Formato: |
application/pdf
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| Fonte: |
Plos One (1932-6203) (Public Library Science), 2013-05 , Vol. 8 , N. 5 , P. -
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| Direitos: |
2013 Genard et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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