| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Quantification of genes expressed during an in vitro infection of haemocytes from Ostrea edulis with parasites Bonamia ostreae by Real times PCR
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| Autores: |
Segarra, Amelie
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| Data: |
2008
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| Ano: |
2008
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| Resumo: |
Oyster fanning represents a significant economic part of the French aquaculture production. This production is approximately about 150 000 tons of shells a year [Buestel, 2004]. However the development ofthis activity can be limited by infectious diseases (virus, protozoan or bacteria). Flat oysters suffered from two epizooties caused by two parasitic di seases: martieilliosis and the bonamiosis. These diseases appeared at the end of the Sixties and Seventies respectively. These diseases had a negative impact on the economy because they decrease the production of Flat oysters by 90% between 1979 and .1980. [Buestel 2004, Grizel 1985] The means of fights against bonamiosis are limited and mainly rely on a preventive approach. In fact, it is impossible to use the vaccination especially because they do not have abodes. But it is also impossible to use treatments for invertebrate because they are product in open systems on the wild. The bonamiosis is a notifiable disease listed by the The World Organisation for Animal Health (OIE) as weil as by European Union. That implies a surveillance of stocks and restrictions of transfer in order to avoid the contamination of healthy zones free. In addition another approach is based on the obtention of resistant animaIs through a genetic selection. Thanks to that it is possible to obtain a better rate of survival in the zones where the parasite is endemic [Naciri et Al.; 1998]. In this context, a better understanding of defence mechanisms developed by the flat oyster against parasite and interaction between oyster and parasite is needed. These data are necessary for the conservation of the flat oyster and to the restoring of flat oyster on our coasts. The objective of this work was to study in vitro the expression of genes of haemocytes of flat oyster in contact with some purified parasite Bonamia ostreae. In a first step, our study has consisted in standardizing the Real Time Quantitative PCR in order to obtain curves standards for quantity the expression of genes of Ostrea edulis. After a session dedicated to bibliographical researches, we will see material and methods presented in the second part. Lastly results will be exposed and discussed.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00368/47909/47939.pdf
http://archimer.ifremer.fr/doc/00368/47909/
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| Formato: |
application/pdf
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| Direitos: |
2008 Ifremer, SupBiotech
info:eu-repo/semantics/openAccess
restricted use
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