| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Serpentinicella alkaliphila gen. nov., sp nov., a novel alkaliphilic anaerobic bacterium isolated from the serpentinite-hosted Prony hydrothermal field, New Caledonia
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| Autores: |
Mei, Nan
Postec, Anne
Erauso, Gael
Joseph, Manon
Pelletier, Bernard
Payri, Claude
Ollivier, Bernard
Quemeneur, Marianne
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| Data: |
2016-11
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| Ano: |
2016
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| Resumo: |
A novel anaerobic, alkaliphilic, Gram-stain-positive, spore-forming bacterium was isolated from a carbonaceous hydrothermal chimney in Prony Bay, New Caledonia. This bacterium, designated strain 3b(T), grew at temperatures from 30 to 43 degrees C (optimum 37 degrees C) and at pH between 7.8 and 10.1 (optimum 9.5). Added NaCl was not required for growth (optimum 0-0.2 %, w/v), but was tolerated at up to 4 %. Yeast extract was required for growth. Strain 3b(T) utilized crotonate, lactate and pyruvate, but not sugars. Crotonate was dismutated to acetate and butyrate. Lactate was disproportionated to acetate and propionate. Pyruvate was degraded to acetate plus trace amounts of hydrogen. Growth on lactate was improved by the addition of fumarate, which was used as an electron acceptor and converted to succinate. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate, nitrite, FeCl3, Fe(III)-citrate, Fe(III)-EDTA, chromate, arsenate, selenate and DMSO were not used as terminal electron acceptors. The G+C content of the genomic DNA was 33.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the isolate is a member of the family Clostridiaceae, order Clostridiales within the phylum Firmicutes. Strain 3b(T) was most closely related to 'Alkaliphilus hydrothermalis' FatMR1(T) (92.2% 16S rRNA gene sequence similarity), and was positioned approximately equidistantly between the genera Alkaliphilus, Anaerovirgula and Natronincola. On the basis of phylogenetic, genetic, chemotaxonomic and physiological properties, strain 3b(T) is proposed to represent a novel species of a new genus, for which the name Serpentinicella alkaliphila gen. nov., sp. nov. is proposed. The type strain of Serpentinicella alkaliphila is 3b(T) (=DSM 100013(T) =JCM 30645(T)).
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00467/57912/60312.pdf
https://archimer.ifremer.fr/doc/00467/57912/60313.pdf
DOI:10.1099/ijsem.0.001375
https://archimer.ifremer.fr/doc/00467/57912/
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| Editor: |
Microbiology Soc
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| Formato: |
application/pdf
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| Fonte: |
International Journal Of Systematic And Evolutionary Microbiology (1466-5026) (Microbiology Soc), 2016-11 , Vol. 66 , P. 4464-4470
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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