| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Functional Screening of Hydrolytic Activities Reveals an Extremely Thermostable Cellulase from a Deep-Sea Archaeon
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| Autores: |
Leis, Benedikt
Heinze, Simon
Angelov, Angel
Pham, Vu Thuy Trang
Thürmer, Andrea
Jebbar, Mohamed
Golyshin, Peter N.
Streit, Wolfgang R.
Daniel, Rolf
Liebl, Wolfgang
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| Data: |
2015-07
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| Ano: |
2015
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| Palavras-chave: |
Functional screenings
Extreme thermostable protein
Archaeal endoglucanase
Enzymatic characterization
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| Resumo: |
Extreme habitats serve as a source of enzymes that are active under extreme conditions and are candidates for industrial applications. In this work, six large-insert mixed genomic libraries were screened for hydrolase activities in a broad temperature range (8–70°C). Among a variety of hydrolytic activities, one fosmid clone, derived from a library of pooled isolates of hyperthermophilic archaea from deep sea vents, displayed hydrolytic activity on carboxymethyl cellulose substrate plates at 70°C but not at lower temperatures. Sequence analysis of the fosmid insert revealed a gene encoding a novel glycoside hydrolase family 12 (GHF12) endo-1,4-β-glucanase, termed Cel12E. The enzyme shares 45% sequence identity with a protein from the archaeon Thermococcus sp. AM4 and displays a unique multidomain architecture. Biochemical characterization of Cel12E revealed a remarkably thermostable protein, which appears to be of archaeal origin. The enzyme displayed maximum activity at 92°C and was active on a variety of linear 1,4-β-glucans like carboxymethyl cellulose, β-glucan, lichenan, and phosphoric acid swollen cellulose. The protein is able to bind to various insoluble β-glucans. Product pattern analysis indicated that Cel12E is an endo-cleaving β-glucanase. Cel12E expands the toolbox of hyperthermostable archaeal cellulases with biotechnological potential.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00601/71319/69744.pdf
https://archimer.ifremer.fr/doc/00601/71319/69745.pdf
https://archimer.ifremer.fr/doc/00601/71319/69746.pdf
DOI:10.3389/fbioe.2015.00095
https://archimer.ifremer.fr/doc/00601/71319/
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| Editor: |
Frontiers Media SA
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| Relação: |
info:eu-repo/grantAgreement/EC/FP7/287589/EU//MICRO B3
info:eu-repo/grantAgreement/EC/FP7/226977/EU//MAMBA
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| Formato: |
application/pdf
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| Fonte: |
Frontiers in Bioengineering and Biotechnology (2296-4185) (Frontiers Media SA), 2015-07 , Vol. 3 , N. 95 , P. 10p.
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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