| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Spectral fluorometric characterization of Haptophyte dynamics using the FluoroProbe: an application in the eastern English Channel for monitoring Phaeocystis globosa
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| Autores: |
Houliez, Emilie
Lizon, Fabrice
Thyssen, Melilotus
Artigas, Luis Felipe
Schmitt, Francois G.
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| Data: |
2012-02
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| Ano: |
2012
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| Palavras-chave: |
Spectral fluorescence
Phaeocystis globosa
Haptophytes
Algal blooms
Phytoplankton quantification
Chlorophyll a
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| Resumo: |
In this study, we examined the possibility of using the FluoroProbe for monitoring the dynamics of the Haptophyte Phaeocystis globosa in the coastal waters of the eastern English Channel. The FluoroProbe was recalibrated by recording a new fingerprint for P. globosa and the use of this new fingerprint was tested through a series of laboratory and in situ experiments. The annual dynamics of P. globosa estimated using the FluoroProbe and by flow cytometry were similar. A strong relationship was found between the FluoroProbe estimates of P. globosa biomass expressed in terms of chlorophyll a equivalent per litre (eq. mu g L(-1)) and flow cytometric cell counts (r = 0.889, P < 0.001, n = 121). The FluoroProbe can be used to detect the flagellated cells as well as the colonial cells of P. globosa but not to distinguish these two cell types in mixed assemblages. The use of the new fingerprint recorded for P. globosa improved the detection of Isochrysis sp. This suggests the possibility of using the FluoroProbe to monitor Haptophytes other than P. globosa by calibrating the device with species representative of the region of interest. However, it is important to note that the detection of P. globosa at the species level was possible in the eastern English Channel because it was the only Haptophyte species present with a biomass sufficient to be detected by the FluoroProbe. In areas where several Haptophyte species are simultaneously present, their discrimination will be impossible and in such situations the FluoroProbe can be used to monitor the dynamics of the combined Haptophyte group.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00373/48461/48723.pdf
DOI:10.1093/plankt/fbr091
https://archimer.ifremer.fr/doc/00373/48461/
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| Editor: |
Oxford Univ Press
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| Formato: |
application/pdf
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| Fonte: |
Journal Of Plankton Research (0142-7873) (Oxford Univ Press), 2012-02 , Vol. 34 , N. 2 , P. 136-151
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| Direitos: |
The Author 2011. Published by Oxford University Press. All rights reserved.
info:eu-repo/semantics/openAccess
restricted use
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