| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Natural abundance of N-15 and C-13 in fish tissues and the use of stable isotopes as dietary protein tracers in rainbow trout and gilthead sea bream
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| Autores: |
Beltran, M.
Fernandez-borras, J.
Medale, Francoise
Perez-sanchez, J.
Kaushik, Sadasivam
Blasco, J.
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| Data: |
2009-02
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| Ano: |
2009
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| Palavras-chave: |
Sea bream
Rainbow trout
Protein
Diet
Delta N 15
Delta C 13
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| Resumo: |
For developing efficient diets, two sets of experiments examined whether the use and allocation of dietary protein can be traced by labelling with stable isotopes (N-15 and C-13) in two culture fish (Oncorhynchus mykiss and Sparus aurata). In the first experiment, natural abundance and tissue distribution of these isotopes were determined, by measuring the delta C-13 and delta N-15 values by isotopic ratio mass spectrometry, in fingerlings (14-17 g) adapted to diets differing in the percentage of fish meal replacement by plant protein sources. For both species, delta N-15 and delta C-13 were greater in tissues with higher protein and lower lipid content. Delta N-15 of diets and tissues decreased as replacement increased, suggesting delta N-15 can be used as a marker for dietary protein origin. The N-15 fractionation (delta N-15 fish - delta N-15 diet) differed between groups, and could thus be used to indicate protein catabolism. In the second experiment, fish (75-90 g) of each species ingested a diet enriched with N-15-protein (10 g kg(-1) diet) and C-13-protein (30 g kg(-1) diet). These proportions were suitable for determining that the delta values of tissue components were high enough above natural levels to allow protein allocation to be traced at 11 and 24 h after feeding, and revealed clear metabolic differences between species.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2009/publication-6966.pdf
DOI:10.1111/j.1365-2095.2008.00563.x
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| Editor: |
Wiley / Blackwell
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| Relação: |
http://archimer.ifremer.fr/doc/00000/6966/
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| Formato: |
application/pdf
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| Fonte: |
Aquaculture Nutrition (1353-5773) (Wiley / Blackwell), 2009-02 , Vol. 15 , N. 1 , P. 9-18
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| Direitos: |
2009 Blackwell Publishing
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