| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Poly I:C induces a protective antiviral immune response in the Pacific oyster (Crassostrea gigas) against subsequent challenge with Ostreid herpesvirus (OsHV-1 mu var)
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| Autores: |
Green, Timothy
Montagnani, Caroline
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| Data: |
2013-08
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| Ano: |
2013
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| Palavras-chave: |
Crassostrea gigas
OsHV-1
Poly I:C
Vibrio splendidus
Interferon-like response
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| Resumo: |
In-vivo studies were carried out to investigate the protective effect of a synthetic viral analogue (poly I:C) against Ostreid herpes virus (OsHV-1 mu var). Pacific oysters (Crassostrea gigas) were immune-primed by intramuscular injection of 240 mu g of poly I:C or sterile seawater at 1 day prior to infection with OsHV-1 mu var. Poly I:C injection induced an antiviral state in C gigas as the percentage of viral-infected oysters at 48 h post infection was significantly lower in the poly I:C treatment (11%) compared to seawater controls (100%). In an additional experiment, we demonstrated that the protective role of poly I:C is reproducible and elicits a specific antiviral response as immune-priming with heat-killed Vibrio splendidus provided no protection against subsequent viral infection. In both experiments, genes homologous to a toll-like receptor (TLR), MyD88, interferon regulatory factor (IRF) and protein kinase R (PKR) were up-regulated in oysters immune-primed with poly I:C compared to seawater controls (p < 0.05). The MyD88, IRF and PKR genes were also significantly up-regulated in response to OsHV-1 mu var infection (p < 0.05), which is suggestive that they are implicated in the antiviral response of C gigas. Our results demonstrate that C gigas can recognise double-strand RNA to initiate an innate immune response that inhibits viral infection. The observed response has striking similarities to the hallmarks of the type-1 interferon response of vertebrates.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00152/26293/24739.pdf
DOI:10.1016/j.fsi.2013.04.051
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| Editor: |
Academic Press Ltd- Elsevier Science Ltd
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| Relação: |
http://archimer.ifremer.fr/doc/00152/26293/
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| Formato: |
application/pdf
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| Fonte: |
Fish & Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2013-08 , Vol. 35 , N. 2 , P. 382-388
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| Direitos: |
2013 Elsevier Ltd. All rights reserved.
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