| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Monitoring of four DNA extraction methods upstream of high-throughput sequencing of Anisakidae nematodes
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| Autores: |
Seesao, Y.
Audebert, C.
Verrez-bagnis, Veronique
Merlin, S.
Jerome, Marc
Viscogliosi, E.
Dei-cas, E.
Aliouat-denis, C. M.
Gay, Melanie
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| Data: |
2014-07
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| Ano: |
2014
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| Palavras-chave: |
Anisakidae
High-throughput sequencing
Nematode
Pooled DNA extraction
Marine fish
COX2
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| Resumo: |
Different methods were evaluated to extract DNA from pooled nematodes belonging to Anisakis, Contracaecum, Pseudoterranova and Hysterothylacium genera isolated from edible fish. Pooled DNA extraction is the first and compulsory step to allow the identification of a large number of samples through high-throughput DNA sequencing with drastic time and cost reductions.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00189/30020/28607.pdf
DOI:10.1016/j.mimet.2014.05.004
https://archimer.ifremer.fr/doc/00189/30020/
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| Editor: |
Elsevier Science Bv
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| Formato: |
application/pdf
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| Fonte: |
Journal Of Microbiological Methods (0167-7012) (Elsevier Science Bv), 2014-07 , Vol. 102 , P. 69-72
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| Direitos: |
2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
info:eu-repo/semantics/openAccess
restricted use
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