| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Long-range PCR and high-throughput sequencing of Ostreid herpesvirus 1 indicate high genetic diversity and complex evolution process
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| Autores: |
Bai, Chang-ming
Morga, Benjamin
Rosani, Umberto
Shi, Jie
Li, Chen
Xin, Lu-sheng
Wang, Chong-ming
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| Data: |
2019-01
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| Ano: |
2019
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| Palavras-chave: |
Long-range PCR
OsHV-1
High-throughput sequencing
Evolution
Diversity
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| Resumo: |
Ostreid herpesvirus 1 (OsHV-1) is an important pathogen associated with mass mortalities of cultivated marine mollusks worldwide. Since no cell line allows OsHV-1 replication in vitro, it is difficult to isolate enough high-purity viral DNA for High-Throughput Sequencing (HTS). We developed an efficient approach for the enrichment of OsHV-1 DNA for HTS with long-range PCR. Twenty-three primer pairs were designed to cover 99.3% of the reference genome, and their performances were examined on ten OsHV-1 infected samples. Amplicon mixtures from six successfully amplified samples were sequenced with Illumina platform, and one of them (ZK0118) was also sequenced with the PacBio platform. PacBio reads were assembled into 2 scaffolds compared to 9−68 scaffolds assembled from the Illumina reads. Genomic comparison confirmed high genetic diversity among OsHV-1 variants. Phylogenetic analysis revealed that OsHV-1 evolution was mainly impacted by its host species rather than spatial segregation.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00464/57566/64184.pdf
DOI:10.1016/j.virol.2018.09.026
https://archimer.ifremer.fr/doc/00464/57566/
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| Editor: |
Academic Press Inc Elsevier Science
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| Formato: |
application/pdf
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| Fonte: |
Virology (0042-6822) (Academic Press Inc Elsevier Science), 2019-01 , Vol. 526 , P. 81-90
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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