| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Molecular diversity and distribution of marine fungi across 130 European environmental samples
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| Autores: |
Richards, Thomas A.
Leonard, Guy
Mahe, Frederic
Del Campov, Javier
Romac, Sarah
Jones, Meredith D. M.
Maguire, Finlay
Dunthorn, Micah
De Vargas, Colomban
Massana, Ramon
Chambouvet, Aurelie
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| Data: |
2015-11
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| Ano: |
2015
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| Palavras-chave: |
454 pyrosequencing
Chytrids
Dikarya
Sediment communities
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| Resumo: |
Environmental DNA and culture-based analyses have suggested that fungi are present in low diversity and in low abundance in many marine environments, especially in the upper water column. Here, we use a dual approach involving high-throughput diversity tag sequencing from both DNA and RNA templates and fluorescent cell counts to evaluate the diversity and relative abundance of fungi across marine samples taken from six European near-shore sites. We removed very rare fungal operational taxonomic units (OTUs) selecting only OTUs recovered from multiple samples for a detailed analysis. This approach identified a set of 71 fungal 'OTU clusters' that account for 66% of all the sequences assigned to the Fungi. Phylogenetic analyses demonstrated that this diversity includes a significant number of chytrid-like lineages that had not been previously described, indicating that the marine environment encompasses a number of zoosporic fungi that are new to taxonomic inventories. Using the sequence datasets, we identified cases where fungal OTUs were sampled across multiple geographical sites and between different sampling depths. This was especially clear in one relatively abundant and diverse phylogroup tentatively named Novel Chytrid-Like-Clade 1 (NCLC1). For comparison, a subset of the water column samples was also investigated using fluorescent microscopy to examine the abundance of eukaryotes with chitin cell walls. Comparisons of relative abundance of RNA-derived fungal tag sequences and chitin cell-wall counts demonstrate that fungi constitute a low fraction of the eukaryotic community in these water column samples. Taken together, these results demonstrate the phylogenetic position and environmental distribution of 71 lineages, improving our understanding of the diversity and abundance of fungi in marine environments.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00374/48490/48880.pdf
https://archimer.ifremer.fr/doc/00374/48490/48887.pdf
https://archimer.ifremer.fr/doc/00374/48490/48888.pdf
https://archimer.ifremer.fr/doc/00374/48490/48889.pdf
https://archimer.ifremer.fr/doc/00374/48490/48890.pdf
https://archimer.ifremer.fr/doc/00374/48490/48891.pdf
https://archimer.ifremer.fr/doc/00374/48490/48892.pdf
DOI:10.1098/rspb.2015.2243
https://archimer.ifremer.fr/doc/00374/48490/
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| Editor: |
Royal Soc
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| Relação: |
info:eu-repo/grantAgreement/EC/FP7/331450/EU//CAARL
info:eu-repo/grantAgreement/EC/FP7/299815/EU//PARAFROGS
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| Formato: |
application/pdf
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| Fonte: |
Proceedings Of The Royal Society B-biological Sciences (0962-8452) (Royal Soc), 2015-11 , Vol. 282 , N. 1819 , P. 1-10
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| Direitos: |
2015 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
info:eu-repo/semantics/openAccess
restricted use
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