| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Genotoxicity and activation of cellular defenses in transplanted zebra mussels Dreissena polymorpha along the Seine river
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| Autores: |
Chatel, Annelle
Faucet-marquis, Virginie
Gourlay-france, Catherine
Pfohl-leszkowicz, Annie
Hubert, Francoise
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| Data: |
2015-04
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| Ano: |
2015
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| Palavras-chave: |
Biomarker
Pollution
DNA adducts
Detoxification
Dreissena polymorpha
RT-PCR
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| Resumo: |
The aim of the present study was to confirm the relevance of studying DNA adduct formation in a field study. In that context, freshwater mussels Dreissena polymorpha, collected in a reference station, were transplanted in different sites with a pollution gradient. After one and two months, mussels were collected and DNA adduct formation was analyzed using the 32P post labelling technique on both gills and digestive glands. In addition, the expression of genes involved in the detoxification system (catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), HSP70, aryl hydrocarbon receptor (AHR), P glycoprotein (PgP), metallothionein (MT)) was assessed by RT-PCR. DNA adducts were observed at amount comparable to data from literature. Increase of DNA adducts after two months of transplantation could be correlated with strong modulation of gene expression implicated in detoxification processes. Indeed, PgP and HSP70 gene expressions were similarly induced in gills and digestive glands while SOD and CAT expressions were down regulated in both tissues. AHR, GST and MT genes were differently regulated depending upon the tissue studied and the level of contamination in the different sites. We demonstrated that mussels transplanted in the different stations with pollution gradient were able to biotransform PAHs, assessed by DNA adduct formation and the high decrease of detoxification genes. Specific DNA adducts pattern obtained after one and two month mussel transplantations demonstrated the relevance of DNA adduct as biomarker of environmental pollution.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00195/30639/32450.pdf
DOI:10.1016/j.ecoenv.2014.03.023
https://archimer.ifremer.fr/doc/00195/30639/
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| Editor: |
Academic Press Inc Elsevier Science
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| Formato: |
application/pdf
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| Fonte: |
Ecotoxicology And Environmental Safety (0147-6513) (Academic Press Inc Elsevier Science), 2015-04 , Vol. 114 , P. 241-249
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| Direitos: |
2014 Elsevier Inc. All rights reserved.
info:eu-repo/semantics/openAccess
restricted use
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