| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Insights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids
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| Autores: |
Batista, Frederico M.
Lopez-sanmartin, Monserrat
Boudry, Pierre
Navas, Jose I.
Ruano, Francisco
Renault, Tristan
Fonseca, Vera G.
Leitao, Alexandra
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| Data: |
2016-05
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| Ano: |
2016
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| Palavras-chave: |
Aneuploidy
Ostreid herpesvirus 1
Oyster
Crassostrea
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| Resumo: |
Cytogenetic abnormalities associated with viral infections, including from viruses of the Herpesvirales order, have been reported in vertebrate species. Ostreid herpesvirus 1 (OsHV-1) has been detected worldwide during mortality outbreaks of the Pacific oyster Crassostrea gigas. On the other hand, a high proportion of aneuploid cells in somatic tissues have been observed in C. gigas. In this study, we analysed the putative association between aneuploidy levels and the detection of OsHV-1 in gills of C. gigas, the Portuguese oyster C. angulata and their F1 hybrids cultured in Ria Formosa (Portugal). OsHV-1 was detected by PCR in 5.4% of the total of oysters analysed (n = 111) namely in 11.1%, 8.0% and 1.7% of C. gigas, C. angulata and F1 hybrid respectively. Sequencing analysis of a viral fragment amplified with the C2/C6 primer pair revealed a high similarity with the OsHV-1 reference type. Moreover, in situ hybridization confirmed the presence of OsHV-1 in gill tissue. Oysters where OsHV-1 was detected had a significantly higher mean percentage of aneuploid cells (25%) than the ones where the virus was not detected (18%). However, the overall low percentage of positive samples contrasted with the high mean percentage of aneuploidy observed, with 50% of the oysters analysed showing a percentage of aneuploid cells between 20% and 30%. We hypothesize that somatic aneuploidy may adversely affect oysters making them more prone to OsHV-1 infection, but the virus is unlikely to be the cause of somatic aneuploidy.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00215/32581/35759.pdf
DOI:10.1111/are.12613
https://archimer.ifremer.fr/doc/00215/32581/
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| Editor: |
Wiley-blackwell
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| Formato: |
application/pdf
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| Fonte: |
Aquaculture Research (1355-557X) (Wiley-blackwell), 2016-05 , Vol. 47 , N. 5 , P. 1530-1536
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| Direitos: |
2014 John Wiley & Sons Ltd
info:eu-repo/semantics/openAccess
restricted use
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