| Registro completo |
| Provedor de dados: |
ArchiMer
|
| País: |
France
|
| Título: |
Optimization of common cuttlefish (Sepia officinalis) protein hydrolysate using Pepsin by Response Surface Methodology
|
| Autores: |
Kechaou, Emna
Berge, Jean-pascal
Jaouen, Pascal
Ben Amar, Raja
|
| Data: |
2015-04
|
| Ano: |
2015
|
| Palavras-chave: |
Cuttlefish protein hydrolysate (CPH)
Amino acid
Enzyme
Sepia officinalis
|
| Resumo: |
Protein hydrolysate was prepared from common cuttlefish Sepia officinalis by Pepsin. Hydrolysis conditions (time, temperature, and enzyme activity) were optimized by response surface methodology (RSM) using a factorial design. The regression coefficient was close to 0.999, observed during both experimental and validation runs, and indicated the validity of prediction model. An enzyme activity to substrate ratio (X1), 0.04 AU/g protein; time (X2), 85 minutes; and temperature (X3), 45 °C were found to be the optimum conditions for a higher degree of hydrolysis (21%) and nitrogen recovery (90%) using Pepsin. According to amino acid analysis results and chemical score, methionine and histidine are the limiting amino acids in the hydrolysates, in that order. All other amino acids are present in sufficient quantity as required.
|
| Tipo: |
Text
|
| Idioma: |
Inglês
|
| Identificador: |
http://archimer.ifremer.fr/doc/00182/29285/27715.pdf
DOI:10.1080/10498850.2013.773116
http://archimer.ifremer.fr/doc/00182/29285/
|
| Editor: |
Taylor & Francis Inc
|
| Formato: |
application/pdf
|
| Fonte: |
Journal Of Aquatic Food Product Technology (1049-8850) (Taylor & Francis Inc), 2015-04 , Vol. 24 , N. 3 , P. 270-282
|
| Direitos: |
Taylor & Francis Group, LLC
info:eu-repo/semantics/openAccess
restricted use
|
|
