| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
The ClC-3 chloride channel and osmoregulation in the European Sea Bass, Dicentrarchus labrax
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| Autores: |
Bossus, Maryline
Charmantier, Guy
Blondeau-bidet, Eva
Valletta, Bianca
Boulo, Viviane
Lorin-nebel, Catherine
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| Data: |
2013-07
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| Ano: |
2013
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| Palavras-chave: |
ClC-3 chloride channel
Na+/K+-ATPase
Dicentrarchus labrax
Osmoregulation
Osmosensing
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| Resumo: |
Dicentrarchus labrax migrates between sea (SW), brackish and fresh water (FW) where chloride concentrations and requirements for chloride handling change: in FW, fish absorb chloride and restrict renal losses; in SW, they excrete chloride. In this study, the expression and localization of ClC-3 and Na+/K+-ATPase (NKA) were studied in fish adapted to SW, or exposed to FW from 10 min to 30 days. In gills, NKA-alpha 1 subunit expression transiently increased from 10 min and reached a stabilized intermediate expression level after 24 h in FW. ClC-3 co-localized with NKA in the basolateral membrane of mitochondria-rich cells (MRCs) at all conditions. The intensity of MRC ClC-3 immunostaining was significantly higher (by 50 %) 1 h after the transfer to FW, whereas the branchial ClC-3 protein expression was 30 % higher 7 days after the transfer as compared to SW. This is consistent with the increased number of immunopositive MRCs (immunostained for NKA and ClC-3). However, the ClC-3 mRNA expression was significantly lower in FW gills. In the kidney, after FW transfer, a transient decrease in NKA-alpha 1 subunit expression was followed by significantly higher stable levels from 24 h. The low ClC-3 protein expression detected at both salinities was not observed by immunocytochemistry in the SW kidney; ClC-3 was localized in the basal membrane of the collecting ducts and tubules 7 and 30 days after transfer to FW. Renal ClC-3 mRNA expression, however, seemed higher in SW than in FW. The potential role of this chloride channel ClC-3 in osmoregulatory and osmosensing mechanisms is discussed.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00149/26044/25185.pdf
DOI:10.1007/s00360-012-0737-9
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| Editor: |
Springer Heidelberg
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| Relação: |
http://archimer.ifremer.fr/doc/00149/26044/
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| Formato: |
application/pdf
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| Fonte: |
Journal Of Comparative Physiology B-biochemical Systemic And Environmental Physiology (0174-1578) (Springer Heidelberg), 2013-07 , Vol. 183 , N. 5 , P. 641-662
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| Direitos: |
Springer-Verlag Berlin Heidelberg 2013
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