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Provedor de dados:  ArchiMer
País:  France
Título:  Molecular cloning and seasonal expression of oyster glycogen phosphorylase and glycogen synthase genes
Autores:  Bacca, Helene
Huvet, Arnaud
Fabioux, Caroline
Daniel, Jean-yves
Delaporte, Maryse
Pouvreau, Stephane
Van Wormhoudt, A
Moal, Jeanne
Data:  2005-04
Ano:  2005
Palavras-chave:  Reproduction
Crassostrea gigas
Resumo:  To investigate the control at the mRNA level of glycogen metabolism in the cupped oyster Crassostrea gigas, we report in the present paper the cloning and characterization of glycogen phosphorylase and synthase cDNAs (Cg-GPH and Cg-GYS, respectively, transcripts of main enzymes for glycogen use and storage), and their first expression profiles depending on oyster tissues and seasons. A strong expression of both genes was observed in the labial palps and the gonad in accordance with specific cells located in both tissues and ability to store glucose. Cg-GPH expression was also found mainly in muscle suggesting ability to use glycogen as readily available glucose to. supply its activity. For seasonal examinations, expression of Cg-GYS and Cg-GPH genes appeared to be regulated according to variation in glycogen content. Relative levels of Cg-GYS transcripts appeared highest in October corresponding to glycogen storage and resting period. Relative levels of Cg-GPH transcripts were highest in May corresponding to mobilization of glycogen needed for germ cell maturation. Expression of both genes would likely be driven by the oyster's reproductive cycle, reflecting the central role of glycogen in energy storage and gametogenic development in C gigas. Both genes are useful molecular markers in the regulation of glycogen metabolism and reproduction in C gigas but enzymatic regulation of glycogen phosphorylase and synthase remains to be elucidated.
Tipo:  Text
Idioma:  Inglês

Editor:  Elsevier
Formato:  application/pdf
Fonte:  Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology (1096-4959) (Elsevier), 2005-04 , Vol. 140 , N. 4 , P. 635-646
Direitos:  2005 Elsevier Inc. All rights reserved

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