| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Direct and fast detection of Alexandrium minutum algae by using high frequency microbalance
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| Autores: |
Sousa, Celia
Compere, Chantal
Dreanno, Catherine
Crassous, Marie-pierre
Gas, Fabienne
Baus, Beatrice
Perrot, Hubert
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| Data: |
2014-09
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| Ano: |
2014
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| Palavras-chave: |
Alexandrium minutum
Quartz microbalance
Microalgae
Immunosensor
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| Resumo: |
In this paper, a simple detection of a toxic algae, Alexandrium minutum, was developed using highly sensitive quartz crystal microbalance. In terms of performance, compared with other conventional analytical tools, the main interest of our immunosensor is based on a fast and direct detection of these living cells. This system requires the use of one monoclonal antibody directed against the surface antigen of A. minutum. We demonstrate that the whole living and motile algae are caught and detected. The high specificity of the biosensor is also demonstrated by testing several other dinoflagellate species. The frequency shift is correlated to the A. minutum cells concentration. This simple system is potentially promising for environmental monitoring purposes.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00194/30492/28897.pdf
DOI:10.1016/j.mimet.2014.05.025
http://archimer.ifremer.fr/doc/00194/30492/
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| Editor: |
Elsevier Science Bv
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| Formato: |
application/pdf
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| Fonte: |
Journal Of Microbiological Methods (0167-7012) (Elsevier Science Bv), 2014-09 , Vol. 103 , P. 49-54
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| Direitos: |
2014 Elsevier B.V. All rights reserved.
info:eu-repo/semantics/openAccess
restricted use
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