| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Detection of griseofulvin in a marine strain of Penicillium waksmanii by ion trap mass spectrometry
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| Autores: |
Petit, Karina
Mondeguer, Florence
Roquebert, M
Biard, J
Pouchus, Y
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| Data: |
2004-07
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| Ano: |
2004
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| Palavras-chave: |
Mass spectrometry ESI IT/MS
Griseofulvin
Antifungal
Marine fungi
Penicillium waksmanii
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| Resumo: |
A marine strain of Penicillium waksmanii Zaleski was isolated from a sample of seawater from shellfish-farming area in the Loire estuary (France). The in vitro marine culture showed an important antifungal activity. Bioassay-guided fractionation was used to purify the crude extract. Dereplication by electrospray-ion trap/mass spectrometry (ESI-IT/MS) afforded the identification of the antifungal compound, after a semi-purification consisting of two stages. A comparison of the ionic composition between the active and the non-active fractions allowed the detection of a monocharged ion at m/z 353 containing a chlorine atom, which could be attributed to the antifungal griseofulvin [C17H17ClO6+H](+). Multi-stage fragmentation (MSn) confirmed the identity of the m/z 353 ion of the antifungal fraction as griseofulvin. It is the first description of griseofulvin production by a strain of P. waksmanii and the first chemical study of a strain of this species isolated from marine temperate cold water.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2004/publication-673.pdf
DOI:10.1016/j.mimet.2004.03.004
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| Editor: |
Elsevier
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| Relação: |
http://archimer.ifremer.fr/doc/00000/673/
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| Formato: |
application/pdf
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| Fonte: |
Journal of Microbiological Methods (0167-7012) (Elsevier), 2004-07 , Vol. 58 , N. 1 , P. 59-65
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| Direitos: |
2004 Elsevier B.V. All rights reserved
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