| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Determination of reduced sulfur compounds by high-performance liquid chromatography in hydrothermal seawater and body fluids from Riftia pachyptila
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| Autores: |
Gru, Cecile
Sarradin, Pierre-marie
Legoff, Hélène
Narcon, Sandrine
Caprais, Jean-claude
Lallier, Francois
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| Data: |
1998-06
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| Ano: |
1998
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| Palavras-chave: |
Sulfide
Sulfite
Thiosulfate
Cysteine
Glutathione
Hydrothermal seawater
Riftia pachyptila
Biological fluids
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| Resumo: |
This paper describes a method for the determination of reduced sulfur compounds in hydrothermal seawater and body fluids from the hydrothermal tube worm Riftia pachyptila. Sulfur is a hey component of the hydrothermal ecosystem based on chemosynthesis, Sulfur compounds were derivatized at pH 8 (4.3 for sulfide in biological fluid) with a fluorescent reagent, monobromobimane, and separated by reverse-phase HPLC. The eluted compounds were detected by spectrofluorimetry. This method allowed: the quantitative analysis of sulfide, sulfite, thiosulfate, cysteine and glutathione in seawater, vascular blood and coelomic fluids from R, pachyptila, The detection limits were in the 0.1 mu mol l(-1) range with a precision lower than 10%, The method has been applied to hydrothermal seawater. The organisms are distributed along a gradient of sulfide (produced by the vent) and thiosulfate. Analysis of biological fluid was performed with a new sample treatment allowing the analysis of total sulfide (free and bound to haemoglobin) with results comparable to published methods.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00186/29763/28203.pdf
DOI:10.1039/a800032h
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| Editor: |
Royal Soc Chemistry
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| Relação: |
http://archimer.ifremer.fr/doc/00186/29763/
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| Formato: |
application/pdf
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| Fonte: |
Analyst (0003-2654) (Royal Soc Chemistry), 1998-06 , Vol. 123 , N. 6 , P. 1289-1293
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