| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts
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| Autores: |
Estevez, Pablo
Sibat, Manoella
Leão-martins, José Manuel
Reis Costa, Pedro
Gago-martínez, Ana
Hess, Philipp
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| Data: |
2020-04
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| Ano: |
2020
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| Palavras-chave: |
Ciguatoxins
HRMS
Q-TOF
Ciguatera poisoning
C-CTX1
Fragmentation pathways
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| Resumo: |
Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the preparation of C-CTX1 laboratory reference material (LRM) from fish species (Seriola fasciata) from the Madeira archipelago (Portugal). This reference material was used to implement a liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) for the detection of C-CTX1, acquisition of full-scan as well as collision-induced mass spectra of this particular analogue. Fragmentation pathways were proposed based on fragments obtained. The optimized LC-HRMS method was then applied to confirm C-CTX1 in fish (Bodianus scrofa) caught in the Selvagens Islands (Portugal).
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00624/73630/73067.pdf
https://archimer.ifremer.fr/doc/00624/73630/73068.pdf
DOI:10.3390/toxins12040267
https://archimer.ifremer.fr/doc/00624/73630/
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| Editor: |
MDPI AG
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| Formato: |
application/pdf
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| Fonte: |
Toxins (2072-6651) (MDPI AG), 2020-04 , Vol. 12 , N. 4 , P. 267 (7p.)
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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