| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Exploring cultivable Bacteria from the prokaryotic community associated with the carnivorous sponge Asbestopluma hypogea
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| Autores: |
Dupont, Samuel
Carre-mlouka, Alyssa
Domart-coulon, Isabelle
Vacelet, Jean
Bourguet-kondracki, Marie-lise
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| Data: |
2014-04
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| Ano: |
2014
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| Palavras-chave: |
Porifera
Asbestopluma hypogea
Carnivorous sponge
Symbiosis
Microbiology
Biologic activities
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| Resumo: |
Combining culture-dependent and independent approaches, we investigated for the first time the cultivable fraction of the prokaryotic community associated with the carnivorous sponge Asbestopluma hypogea. The heterotrophic prokaryotes isolated from this tiny sponge were compared between specimens freshly collected from cave and maintained in aquarium. Overall, 67 isolates obtained in pure culture were phylogenetically affiliated to the bacterial phyla Proteobacteria, Bacteroidetes, Actinobacteria, and Firmicutes. This cultivable diversity was lower than the prokaryotic diversity obtained by previous pyrosequencing study and comparable to that of another Mediterranean demosponge, the filter-feeding Phorbas tenacior. Furthermore, using fluorescence in situ hybridization, we visualized bacterial and archaeal cells, confirming the presence of both prokaryotes in A.hypogea tissue. Approximately 16% of the bacterial isolates tested positive for chitinolytic activity, suggesting potential microbial involvement in the digestion processes of crustacean prey by this carnivorous sponge. Additionally, 6% and 16% of bacterial isolates revealed antimicrobial and antioxidant activities, respectively. One Streptomyces sp. S1CA strain was identified as a promising candidate for the production of antimicrobial and antioxidant secondary metabolites as well as chitinolytic enzymes. Implications in the context of the sponge biology and prey-feeding strategy are discussed.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00372/48273/48572.pdf
DOI:10.1111/1574-6941.12279
https://archimer.ifremer.fr/doc/00372/48273/
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| Editor: |
Oxford Univ Press
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| Formato: |
application/pdf
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| Fonte: |
Fems Microbiology Ecology (0168-6496) (Oxford Univ Press), 2014-04 , Vol. 88 , N. 1 , P. 160-174
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| Direitos: |
2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved
info:eu-repo/semantics/openAccess
restricted use
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