| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
In situ QCM DNA-biosensor probe modification
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| Autores: |
Lazerges M, M
Perrot, H
Zeghib N, N
Antoine, Elisabeth
Compere, Chantal
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| Data: |
2006-12
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| Ano: |
2006
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| Palavras-chave: |
QCM
DNA
Biosensor
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| Resumo: |
We report on a probe modification of a quartz crystal microbalance (QCM) DNA-biosensor that permits to reversibly change the DNA sequence detected. A QCM DNA-biosensor was designed by immobilization of a 20-base DNA-disulfide probe on the gold-covered quartz surface of a 27 MHz microbalance (9 MHz, third overtone). After immobilization on the gold covered quartz surface, this probe was modified by hybridization with a 45-base DNA that includes the complementary 20-base sequence, a 5-base spacer and a non-complementary 20-base sequence. The non-complementary sequence constitutes a new probe, different from the DNA-disulfide probe, that permits the detection of a new DNA target. As this 45 bases DNA is changeable by dehybridization, successive different DNA targets can be detected. Kinetics and thermodynamic studies of the DNA-disulfide and modified biosensors indicate that the modified biosensor is as sensitive, selective, fast, renewable and reproducible as the DNA-disulfide biosensor, but with a higher hybridization ratio. This modification method offers wider investigation field and practical and economic advantages to DNA-biosensors based on irreversible immobilization of DNA probes on solid substrate. (c) 2006 Elsevier B.V. All rights reserved.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2006/publication-1985.pdf
DOI:10.1016/j.snb.2006.02.024
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| Editor: |
Elsevier
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| Relação: |
http://archimer.ifremer.fr/doc/00000/1985/
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| Formato: |
application/pdf
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| Fonte: |
Sensors and Actuators B: Chemical (0925-4005) (Elsevier), 2006-12 , Vol. 120 , N. 1 , P. 329-337
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| Direitos: |
2006 Elsevier B.V.
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