| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Molecular identification of Vibrio tapetis, the causative agent of the brown ring disease of Ruditapes philippinarum
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| Autores: |
Paillard, Christine
Gausson, Sabrina
Nicolas, Jean-louis
Le Pennec, Jean Paul
Haras, Dominique
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| Data: |
2006-03
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| Ano: |
2006
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| Palavras-chave: |
DOT BLOT hybridisation
SSP PCR
Vibriosis
Bivalve
Detection
Diagnostic
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| Resumo: |
Vibrio tapetis is the marine bacterium responsible for the brown ring disease (BRD) affecting die manila clam, Ruditapes philippinarum. Identification of V tapelis has been previously performed using biochemical criteria and serological procedures. All of these methods are time consuming and ill-adapted to individual screening. This study describes an oligonucleotidic probe (Vt446) and two PCR primers, deduced from the 16S rDNA sequence, allowing a fast and specific V tapetis identification using dot blot hybridisation and species-specific primed PCR (SSP-PCR). The probe and primers have been tested on 60 strains, including referenced Vibrio sp., Gram negative and positive bacteria, marine bacteria samples and isolated clam bacteria. For all the 19 V tapetis strains, the results of PCR assays consistently corroborated those of the agglutination tests. The detection limit was estimated to be 10(2) CFU ml(-1). The SSP-PCR method has resulted in V tapetis detection in larvae, m diseased clams, and in asymptomatic broodstock clams that later developed BRD. In conclusion, the two SSP-PCR primers were useful for direct and fast identification of V tapetis strains isolated in clams, and are well suited for the screening of individual R. philippilnarum broodstock clams and larvae from the hatchery. (C) 2005 Elsevier B.V. All rights reserved.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2006/publication-1692.pdf
DOI:10.1016/j.aquaculture.2005.03.047
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| Editor: |
Elsevier
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| Relação: |
http://archimer.ifremer.fr/doc/00000/1692/
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| Formato: |
application/pdf
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| Fonte: |
Aquaculture (0044-8486) (Elsevier), 2006-03 , Vol. 253 , N. 1-4 , P. 25-38
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| Direitos: |
2005 Elsevier B.V. All rights reserved
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