| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
A flavoprotein supports cell wall properties in the necrotrophic fungus Alternaria brassicicola
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| Autores: |
Pigné, Sandrine
Zykwinska, Agata
Janod, Etienne
Cuenot, Stéphane
Kerkoud, Mohammed
Raulo, Roxane
Bataillé-simoneau, Nelly
Marchi, Muriel
Kwasiborski, Anthony
N’guyen, Guillaume
Mabilleau, Guillaume
Simoneau, Philippe
Guillemette, Thomas
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| Data: |
2017-12
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| Ano: |
2017
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| Palavras-chave: |
Monooxygenase
Cell wall
Melanin
Fungus
Flavoprotein
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| Resumo: |
Background: Flavin-dependent monooxygenases are involved in key biological processes as they catalyze a wide variety of chemo-, regio- and enantioselective oxygenation reactions. Flavoprotein monooxygenases are frequently encountered in micro-organisms, most of which require further functional and biocatalytic assessment. Here we investigated the function of the AbMak1 gene, which encodes a group A flavin monooxygenase in the plant pathogenic fungus Alternaria brassicicola, by generating a deficient mutant and examining its phenotype. Results: Functional analysis indicates that the AbMak1 protein is involved in cell wall biogenesis and influences the melanization process. We documented a significant decrease in melanin content in the Äabmak1 strain compared to the wild-type and complemented strains. We investigated the cell wall morphology and physical properties in the wild-type and transformants using electron and atomic force microscopy. These approaches confirmed the aberrant morphology of the conidial wall structure in the Äabmak1 strain which had an impact on hydrophilic adhesion and conidial surface stiffness. However, there was no significant impairment in growth, conidia formation, pathogenicity or susceptibility to various environmental stresses in the Äabmak1 strain. Conclusion: This study sheds new light on the function of a fungal flavin-dependent monooxygenase, which plays an important role in melanization.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00371/48189/48305.pdf
https://archimer.ifremer.fr/doc/00371/48189/48307.tiff
https://archimer.ifremer.fr/doc/00371/48189/48308.png
https://archimer.ifremer.fr/doc/00371/48189/48309.tiff
https://archimer.ifremer.fr/doc/00371/48189/48310.pdf
DOI:10.1186/s40694-016-0029-3
https://archimer.ifremer.fr/doc/00371/48189/
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| Editor: |
Springer Nature
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| Formato: |
application/pdf
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| Fonte: |
Fungal Biology and Biotechnology (2054-3085) (Springer Nature), 2017-12 , Vol. 4 , N. 1 , P. 1-13
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| Direitos: |
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) ap
info:eu-repo/semantics/openAccess
restricted use
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