| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Monoclonal antibodies against Bonamia ostreae (Protozoa: Ascetospera), an intrahaemocytic parasite of flat oyster Ostrea edulis (Mollusca : Bivlavia)
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| Autores: |
Rogier, Hervé
Hervio, Dominique
Boulo, Viviane
Clavies, Christine
Hervaud, Eliane
Bachere, Evelyne
Mialhe, Eric
Grizel, Henri
Pau, Bernard
Paolucci, Francis
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| Data: |
1991-08
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| Ano: |
1991
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| Palavras-chave: |
Monoclonal antibodies
Oyster
Ostrea edulis
Bonamia ostreae
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| Resumo: |
The protozoan Bonamia ostreae (Ascetospora), a paasite of the flat oyster Ostrea edulis, was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibiodies identified. One of these antibodies (20B2-1B12) has an apparent association constant of ca 3 X 10(8) M-1 and bound to a number of different sites on the parasite. These monoclonal antibodies should prove to be of great value as diagnostic and research tools.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/1991/publication-2756.pdf
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| Editor: |
Inter-Research
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| Relação: |
http://archimer.ifremer.fr/doc/00000/2756/
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| Formato: |
application/pdf
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| Fonte: |
Diseases of aquatic organisms (Inter-Research), 1991-08 , Vol. 11 , P. 135-142
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| Direitos: |
Inter-Research
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