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Provedor de dados:  BABT
País:  Brazil
Título:  Investigation of natural infection by Leishmania in sandflies of Paraná State, Southern Brazil
Autores:  Scodro,Regiane Bertin de Lima
Reinhold-Castro,Kárin Rosi
Dias-Sversutti,Alessandra de Cassia
Neitzke-Abreu,Herintha Coeto
Membrive,Norberto de Assis
Kühl,João Balduíno
Silveira,Thaís Gomes Verzignassi
Teodoro,Ueslei
Data:  2008-06-01
Ano:  2008
Palavras-chave:  Cutaneous leishmaniasis
Sandflies
Dissection
Polymerase chain reaction
Resumo:  The purpose of this work was to verify the occurrence of Leishmania in naturally infected sandflies. The insects were collected with Falcão, Shannon and HP light-traps, in Doutor Camargo and Maringá municipalities between November 2004 and October 2005. Of the 11,033 sandflies collected in Doutor Camargo, 2,133 surviving females were dissected, particularly those of the Nyssomyia neivai species (86.87%). In Maringá, 136 sandflies were collected, of which 79 N. whitmani females and 1 Migonemyia migonei female were dissected. The dissected insects were identified and stored in the pools of 10 specimens. The PCR was carried out on 1,190 females of N. neivai and 190 of N. whitmani from Doutor Camargo, and on 30 of N. whitmani from Maringá, using the primers MP1L/MP3H. The natural infection by Leishmania in sandflies was not confirmed by either of the methods used. The results suggested the low natural infection rate of sandflies by Leishmania in these areas, corroborating other studies carried out in endemic areas of ACL.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300007
Editor:  Instituto de Tecnologia do Paraná - Tecpar
Relação:  10.1590/S1516-89132008000300007
Formato:  text/html
Fonte:  Brazilian Archives of Biology and Technology v.51 n.3 2008
Direitos:  info:eu-repo/semantics/openAccess
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