Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados BABT (9) Autor Souza,Roberto Rodrigues de (9) Santana,José Carlos Curvelo (8) Tambourgi,Elias Basile (8) Lopes,Francisco Luiz Gumes (4) Biazus,Joana Paula Menezes (2) Severo Júnior,João Baptista (2) Barros,Kleber Vanio Gomes (1) Cerqueira,Keilla Santos (1) Coêlho,Diego de Freitas (1) Ehrhardt,Daniela Diniz (1) Evangelista,Alex Ferreira (1) Ferreira,Graziela Batista (1) Ferreira,Juliana Ferrari (1) Franco,Telma Teixeira (1) Jordão,Elizabete (1) Leite,Nadjma Souza (1) Lima,Aloísio André Bomfim de (1) Lédo,Ana da Silva (1) Mazzola,Priscila Gava (1) Márquez,Jesus Espinoza (1) Mais... Palavra-chave Bromelain (3) Optimization (3) Ananas comosus (2) Alpha and beta -amylases enzymes (1) Alpha and beta amylase (1) Amberlite IRA 410 ion-exchange resin (1) Aqueous two-phase systems (1) BSA (1) Bioactive Compounds (1) Biochemistry characterization (1) Breakthrough curves (1) CaCl2 (1) Corn malt (1) Distribution residence time study (1) Enzymatic activity (1) Flat membrane (1) Germination model (1) Green Products (1) Hollow fiber membrane (1) Imperial pineapple (1) Mais... Tipo do documento Info:eu-repo/semantics/article (9) Ano 2019 (1) 2012 (2) 2009 (3) 2007 (2) 2005 (1) País Brazil (9) Idioma Inglês (9)		Registro completo Provedor de dados:  BABT País:  Brazil Título:  Partitioning optimization of proteins from Zea mays malt in ATPS PEG 6000/CaCl2 Autores:  Ferreira,Graziela Batista Evangelista,Alex Ferreira Severo Junior,João Baptista Souza,Roberto Rodrigues de Santana,José Carlos Curvelo Tambourgi,Elias Basile Jordão,Elizabete Data:  2007-05-01 Ano:  2007 Palavras-chave:  Partitioning Optimization Aqueous two-phase systems Maize malt PEG 6000 CaCl2 Resumo:  This work aimed to establish the relationship between the compositions and pH of ATPS PEG 6000/CaCl2 and the proteins partition from maize malt and also to simplify the process optimization in ATPS for a statistical model, established by response surface methodology (RSM). Results showed that these were no influence of pH on the phase diagrams and on the composition of tie line length of PEG 6000/CaCl2 ATPS. SRM analyses showed that elevated pH and larger tie line length were the best conditions for recovering of maize malt proteins. The maximum partition coefficient by PEG 6000/CaCl2 ATPS was about 4.2 and was achieved in ATPS in a single purification step. The theoretical maximum partition coefficient was between 4.1-4.3. The process was very suitable for continuous aqueous two-phase purification due to the stability of proteins (e.g. and -amylases) and could increase their content into middle. Tipo:  Info:eu-repo/semantics/article Idioma:  Inglês Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300020 Editor:  Instituto de Tecnologia do Paraná - Tecpar Relação:  10.1590/S1516-89132007000300020 Formato:  text/html Fonte:  Brazilian Archives of Biology and Technology v.50 n.3 2007 Direitos:  info:eu-repo/semantics/openAccess Fechar

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco