Registro completo |
Provedor de dados: |
BABT
|
País: |
Brazil
|
Título: |
Use of chitosan gel for the purification of protein
|
Autores: |
Tang,ZhenXing
Qian,JunQing
|
Data: |
2007-03-01
|
Ano: |
2007
|
Palavras-chave: |
Chitosan
Gel
Preparation
Application
|
Resumo: |
The aim of this study was to optimize the preparation of chitosan gel and to use it for protein purification. The optimized preparation parameters were chitosan concentration 2.0%, glutaraldehyde concentration 0.6%, low cross-linking rate, NaOH concentration 1.6%, amount of NaBH4 0.4 g. In order to use the chitosan gel, the elution conditions were optimized as follows. NaCl concentration 0.05 mol/L in the tris-HCl (pH 9.05) at the flow rate of 2.03.0 mL/min. Particle size of chitosan gel was 120-140 µm. Neutral protease could be separated into four ingredients through chitosan gel column. The yield of enzyme was more than 90%. Albumin bovine serum could be separated into two ingredients through gel column and the total yield of albumin bovine serum was more than 70%.
|
Tipo: |
Info:eu-repo/semantics/article
|
Idioma: |
Inglês
|
Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200015
|
Editor: |
Instituto de Tecnologia do Paraná - Tecpar
|
Relação: |
10.1590/S1516-89132007000200015
|
Formato: |
text/html
|
Fonte: |
Brazilian Archives of Biology and Technology v.50 n.2 2007
|
Direitos: |
info:eu-repo/semantics/openAccess
|