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Provedor de dados:  BABT
País:  Brazil
Título:  Use of chitosan gel for the purification of protein
Autores:  Tang,ZhenXing
Qian,JunQing
Data:  2007-03-01
Ano:  2007
Palavras-chave:  Chitosan
Gel
Preparation
Application
Resumo:  The aim of this study was to optimize the preparation of chitosan gel and to use it for protein purification. The optimized preparation parameters were chitosan concentration 2.0%, glutaraldehyde concentration 0.6%, low cross-linking rate, NaOH concentration 1.6%, amount of NaBH4 0.4 g. In order to use the chitosan gel, the elution conditions were optimized as follows. NaCl concentration 0.05 mol/L in the tris-HCl (pH 9.05) at the flow rate of 2.03.0 mL/min. Particle size of chitosan gel was 120-140 µm. Neutral protease could be separated into four ingredients through chitosan gel column. The yield of enzyme was more than 90%. Albumin bovine serum could be separated into two ingredients through gel column and the total yield of albumin bovine serum was more than 70%.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200015
Editor:  Instituto de Tecnologia do Paraná - Tecpar
Relação:  10.1590/S1516-89132007000200015
Formato:  text/html
Fonte:  Brazilian Archives of Biology and Technology v.50 n.2 2007
Direitos:  info:eu-repo/semantics/openAccess
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