| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Enhanced Production of Vitamin K2 from Bacillus subtilis (natto)by Mutation and Optimization of the Fermentation Medium
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| Autores: |
Song,Junying
Liu,Hongxia
Wang,Li
Dai,Jun
Liu,Yan
Liu,Hui
Zhao,Genhai
Wang,Peng
Zheng,Zhiming
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| Data: |
2014-08-01
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| Ano: |
2014
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| Palavras-chave: |
Bacillus subtilis (natto)
Mutant
Optimization
Vitamin K2
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| Resumo: |
The aim of this study was to enhance the production of vitamin K2 by using N-methyl-N-nitro-N-nitroso-guanidine (NTG) and low energy ion beam implantation and optimizing the fermentation medium. Mutation resulted in 1.66-fold higher production of vitamin K2 than that of the parentl strain. The production by the mutant BN-P15-11-1was increased 55% and reached 3.593±0.107 mg/L by using the Plackett-Burman and Box-Behnken designs to optimize the fermentation medium. The optimal fermentation culture medium was composed of (g/L) glycerol 69.6, sucrose 34.5, K2HPO4 4.0, peptone 20, yeast extract 25 and fermented at 37 °C and 150 rpm for 72 h. The results showed that the NTG and low energy ion beam implantation mutations and optimizing fermentation medium were effective methods to enhance vitamin K2production.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400606
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-8913201402126
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.57 n.4 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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