| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Concentration by membrane separation processes of a medicinal product obtained from pineapple pulp
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| Autores: |
Lopes,Francisco Luiz Gumes
Severo Júnior,João Baptista
Souza,Roberto Rodrigues de
Ehrhardt,Daniela Diniz
Santana,José Carlos Curvelo
Tambourgi,Elias Basile
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| Data: |
2009-04-01
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| Ano: |
2009
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| Palavras-chave: |
Ananas comosus
Bromelain
Membrane separation processes
Purification
Medicinal product
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| Resumo: |
The concentration of pineapple juice is needed to retain the bromelain activity and to standardize the composition and proteolytic activity. Thus, this work aimed to obtain a pure bromelain extract from the Ananas comosus L. Merril juice by membrane separation process. A 2² experimental planning was used to study the influence of pH and transmembrane pressure on the activity recovery by micro-filtration using a plain membrane. In second step, this enzyme was purified by the ultra-filtration using a 10 kDa millipore kit. The best operation condition to bromelain concentration using the plain membrane was at pH 7.5 and transmembrane pressure of 0.05 bar, while 85% of bromelain activity was recovered. Ultra-filtration retained 100% of proteolytic activity and concentrated in 10 fold the bromelain extract. SDS-PAGE electrophoresis showed that the ultra-filtrated had high purity and the bromelain from A. comosus pulp had a molecular weight of 24.5 kDa.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200024
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132009000200024
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.52 n.2 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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