| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahl
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| Autores: |
Simões,Claudia
Albarello,Norma
Callado,Cátia Henriques
Castro,Tatiana Carvalho de
Mansur,Elisabeth
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| Data: |
2010-06-01
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| Ano: |
2010
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| Palavras-chave: |
Auxin
Embriogenic callus
Histology
Phytagel
Somatic embryos
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| Resumo: |
This paper describes a protocol for the efficient vegetative propagation of Cleome rosea by somatic embryogenesis. Leaf and stem explants from nursery-grown seedlings of C. rosea were cultivated on Murashige and Skoog (MS) medium supplemented with indole-3-acetic acid (IAA), a -naphthaleneacetic acid (NAA), 4-amino-3,5,6-trichloropicolinic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D). Nodular calli were produced from both explant types in the presence of 4.5 and 9.0 µM 2,4-D. Embryo development and maturation were achieved when calli from stem explants were transferred to media containing a ten-fold reduction of 2,4-D concentration initially used (0.45 and 0.90 µM). Leaf-derived calli did not form embryos with the same treatments. The highest frequency of embryogenic callus formation (85%) and number of embryo per callus (13.45 ± 2.8) were achieved during the first subculture on medium supplemented with 0.90 µM 2,4-D. Embryo conversion into plantlets was achieved following transfer to growth regulator-free MS medium solidified with 2 g.L-1 phytagel. An acclimatization rate of 53% was found three months after transfer to ex vitro conditions and the recovered plants presented a normal phenotypic aspect.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300024
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132010000300024
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.53 n.3 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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