| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313
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| Autores: |
Palit,Soumen
Banerjee,Rintu
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| Data: |
2001-03-01
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| Ano: |
2001
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| Palavras-chave: |
Bacillus circulans
Solid state fermentation
Alpha- amylase
Extraction
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| Resumo: |
The extraction of alpha-amylase in the solid state fermentation of wheat bran by Bacillus circulans GRS313 was optimized. Among various solvents tested, maximum extraction was achieved when 2.5% (v/v) glycerol was added. The optimum conditions for extraction were 2.5 hrs soaking time at 30° C under recirculation and agitated condition, which yielded 590 U/g bran of enzyme activity. Whereas under stationary soaking condition the activity of the enzyme was 439.58U/g bran. With repeated wash under the above optimum conditions showed that 600 U/g and 184.2 U/g of enzyme extracted in the 1st and 2nd washes respectively and only 36.84 U/g was recovered in the 3rd wash
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100015
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132001000100015
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.44 n.1 2001
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| Direitos: |
info:eu-repo/semantics/openAccess
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