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Provedor de dados:  BABT
País:  Brazil
Título:  Induction of cassava somatic embryogenesis in liquid medium associated to floating membrane rafts
Autores:  Takahashi,Elizabete Keiko
Kobayashi,Adilson Kenji
Vieira,Luiz Gonzaga Esteves
Data:  2000-01-01
Ano:  2000
Palavras-chave:  Manihot esculenta
Somatic embryogenesis
Membrane rafts
Organized embryogenic structures (OES)
Resumo:  The objective of this study was to examine the effect of two culture systems, liquid medium associated to floating membranes and solid medium, both supplemented with different concentrations of 2,4-D, in the induction of somatic embryogenesis of cassava (Manihot esculenta Crantz). Only 28% of the young leaf lobes (with 9 µM 2,4-D) were induced to form organized embryogenic structures (OES) with membrane rafts, compared to 50% of the explants presenting this type of tissue in solid medium with 36 µM of 2,4-D. Despite the lower response observed in liquid medium with membrane, the amount of OES/explant in all 2,4-D concentrations was higher than solid medium. Based on the results and considering the high cost of the membrane rafts, this system was not distinctly superior than solid medium for inducing somatic embryogenesis in cassava.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100005
Editor:  Instituto de Tecnologia do Paraná - Tecpar
Relação:  10.1590/S1516-89132000000100005
Formato:  text/html
Fonte:  Brazilian Archives of Biology and Technology v.43 n.1 2000
Direitos:  info:eu-repo/semantics/openAccess
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