| Registro completo |
| Provedor de dados: |
BABT
|
| País: |
Brazil
|
| Título: |
Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae)
|
| Autores: |
Klemz,Claudio
Salvo,Ligia Maria
Bastos Neto,Jayme da Cunha
Bainy,Afonso Celso Dias
Assis,Helena Cristina da Silva de
|
| Data: |
2010-04-01
|
| Ano: |
2010
|
| Palavras-chave: |
Cytochrome P450
Fish
Ancistrus multispinis
Biomarker
|
| Resumo: |
Sensitive biological responses to environmental contaminants are useful as early warning signals to predict the damages by long-term exposure. Protocols standardization to quantify biochemical parameters in different fish species is required to validate its use as biomarkers. Comparative studies from different fish species and its interpretation are a challenge for the validation of its use as general biomarkers, representative of environmental impact. In this study, the protocol for liver cytochrome P450 (CYP) analysis from the native Brazilian fish Ancistrus multispinis was established. The microsome contamination by hemoglobin during the analysis of CYP in liver was detected, leading to misinterpretation of the results. The spectrophotometric method for CYP analysis was adapted in order to diminish the hemoglobin interference. Additionally, the western blotting method for CYP1A analysis was tested with success for this fish species.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200015
|
| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
|
| Relação: |
10.1590/S1516-89132010000200015
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Archives of Biology and Technology v.53 n.2 2010
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
