| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Quantitative differential expression of alpha and beta ryanodine receptor genes in PSE (Pale, Soft, Exudative) meat from two chicken lines: broiler and layer
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| Autores: |
Oda,Sandra Helena Inoue
Nepomuceno,Alexandre Lima
Ledur,Mônica Corrêa
Oliveira,Maria Cristina Neves de
Marin,Silvana Regina Rockenbach
Ida,Elza Iouko
Shimokomaki,Massami
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| Data: |
2009-12-01
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| Ano: |
2009
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| Palavras-chave: |
Malignant hyperthermia
Poultry
Gene expression
Real time PCR
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| Resumo: |
Total RNA isolated from Pectoralis major muscle from PSE (L*24h>53.0, pH<5.8) and non-PSE (44<"L*24h>"53) meats of two phenotypically distinct chicken lines, broiler and layer, was used to investigate the α-ryr and β-ryr gene expression by real-time RT-PCR approach. Mean relative quantification (RQ) values were lower (p<0.05) for β-ryr in PSE chickens from both lines when compared to non-PSE chickens, while there was no difference (p>0.05) in α-ryr gene expression regardless of line studied. The β-ryr RQ results suggested that in PSE samples an alteration might occur in the regular ratio (1:1) of α-RyR/β-RyR normally found in avian muscles. These results provided the first evidence of PSE meat occurrence as a result of the differential expression of ryanodine receptor genes which might lead to an increased in Ca2+ availability at the cell milieu.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600024
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132009000600024
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.52 n.6 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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