| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Batch and continuous extraction of bromelain enzyme by reversed micelles
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| Autores: |
Fileti,Ana Maria Frattini
Fischer,Gilvan Anderson
Santana,José Carlos Curvelo
Tambourgi,Elias Basile
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| Data: |
2009-10-01
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| Ano: |
2009
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| Palavras-chave: |
Reversed micelles
Liquid-liquid extraction
Bromelain
Pineapple
Optimization
Surface response
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| Resumo: |
The main aim of this study was to optimize the conditions for bromelain extraction by reversed micelles from pineapple juice (Ananas comosus). The purification was carried out in batch extraction and a micro-column with pulsed caps for continuous extraction. The cationic micellar solution was made of BDBAC as a surfactant, isooctane as a solvent and hexanol as a co-solvent. For the batch process, a purification factor of 3 times at the best values of surfactant agent, co-solvent and salt concentrations, pH of the back and forward extractions were, 100 mM, 10% v/v, 1 M, 3.5 and 8, respectively. For the continuous operation, independent variables optimal point was determined: ratio between light phase flow rate and total flow rate equal to 0.67 and 1 second for the time interval between the pulses. This optimal point led to a productivity of 1.29 mL/min and a purification factor of 4.96.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500021
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132009000500021
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.52 n.5 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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