| Registro completo |
| Provedor de dados: |
BABT
|
| País: |
Brazil
|
| Título: |
Early somatic embryogenesis in Heliconia chartacea Lane ex Barreiros cv. Sexy Pink ovary section explants
|
| Autores: |
Ulisses,Cláudia
Camara,Terezinha Rangel
Willadino,Lilia
Albuquerque,Cynthia Cavalcanti de
Brito,Júlio Zoé de
|
| Data: |
2010-02-01
|
| Ano: |
2010
|
| Palavras-chave: |
Callogenesis
Growth regulator
Histology
Scanning electron microscopy
|
| Resumo: |
The present work evaluated the development of embryogenic callus from transversal ovary sections. The experiments were carried out under two experimental regimes using combinations of IAA (0; 5.71; 8.56; 11.42; 14.27μM) and 2,4-D (0; 13.57; 18.10; 22.62μM) or combinations of 2,4-D with BA (0; 4.43; 6.65; 8.87; 11.09μM). Assessments were made of anatomical aspects of the callus and for the presence of embryogenic structures using cytochemical and histological analyses and stereomicroscopic and scanning electronic microscopic observations. Treatments with 2,4-D and IAA produced friable calluses demonstrating cellular acquisition of morphogenetic competence as well as the formation of pro-embryogenic sectors. The expression of embryogenic program could be observed, with proembryogenic cell clusters developing into globular embryos. These results offer the possibility of using new types of explants for culturing helicons that avoid the growth of endophytic bacteria.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100002
|
| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
|
| Relação: |
10.1590/S1516-89132010000100002
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Archives of Biology and Technology v.53 n.1 2010
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
