| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation
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| Autores: |
Rahulan,Raji
Manjunath,G
Nampoothiri,Kesavan Madhavan
Pandey,Ashok
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| Data: |
2014-04-01
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| Ano: |
2014
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| Palavras-chave: |
Streptomyces gedanensis
Methionine amino peptidase
Fermentation
Box Behnken design
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| Resumo: |
A bioprocess was developed for extracellular MAP production from Streptomyces gedanensis by solid-state fermentation. Response surface methodology of Box Behken Design was performed to evaluate the interaction effects of most significant variables {inoculum size, (NH4)2SO4 concentration, MgSO4.7H2O and tryptone) on MAP production after the single parameter optimization and it resulted a maximum MAP production of 55.26 IU/g PUF after 120 h of fermentation. The concentrated crude MAP displayed a pH and temperature optimum of 8.5 and 50°C. By analyzing the thermal stability, the MAP was found to be stable in a temperature range of 50 to 55°C but lost about 50% of its activity at 65°C after 30 min. This is a first report of this kind of study for MAP.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200005
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132014000200005
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.57 n.2 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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