| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Production of specific polyclonal antibodies anti-human factor IX
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| Autores: |
Chaves,Daniel Gonçalves
Rodrigues,Cibele Velloso
Ferreira,Wanderley Almeida
Siqueira,Pollyana Fantini Miranda
Duarte,Clara Guerra
Carneiro-Proietti,Anna Bárbara de Freitas
Santoro,Marcelo Matos
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| Data: |
2006-07-01
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| Ano: |
2006
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| Palavras-chave: |
Factor IX
Hemophilia B
HPLC
Purification
Antibodies
ELISA
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| Resumo: |
In this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to anionic exchange chromatographic process and with a large yield. Immunoassays (ELISA) using bovine serum albumin, trypsin and peptides generated by cleavage assays with trypsin as digestion enzyme was performed and revealed adequate specificity of the polyclonal antibodies produced.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500010
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132006000500010
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.49 n.4 2006
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| Direitos: |
info:eu-repo/semantics/openAccess
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