| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Genetic variability in regenerated Metarhizium flavoviride protoplasts
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| Autores: |
Kuklinsky-Sobral,Júlia
Luna-Alves-Lima,Elza Áurea de
Araújo,Janete Magali de
Azevedo,João Lúcio
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| Data: |
2004-03-01
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| Ano: |
2004
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| Palavras-chave: |
Metarhizium flavoviride
Protoplasts
Genetic variability
Benomyl
Captan
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| Resumo: |
Protoplast isolation and regeneration were evaluated in two wild-type and two colour mutant strains of Metarhizium flavoviride. Cultivation in liquid medium, followed by mycelium treatment with Novozym 234 in the presence of KCl 0.7M as osmotic stabilizer, produced 5.05 x 10(6) to 1.15 x 10(7)x mL-1 protoplasts. The percentage of regeneration ranged from 6.65 to 27.92%. Following protoplast regeneration, one strain produced spontaneously stable morphological variant colonies. Although colonies with altered morphology have been reported in bacteria following protoplast regeneration, this is the first time that the same is described in a filamentous fungus. The original strain and one derived variant were tested for sensitivity to the fungicides benomyl and captan.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100001
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132004000100001
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.47 n.1 2004
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| Direitos: |
info:eu-repo/semantics/openAccess
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