| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Drifter technique: a new method to obtain metaphases in Hep-2 cell line cultures
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| Autores: |
Lima,Eleonidas Moura
Rissino,Jorge Dores
Guimarães,Adriana Costa
Overal,David James
Khayat,André Salim
Souza,Patrícia Carvalho de
Christofolini,Denise Maria
Smith,Marília de Arruda Cardoso
Burbano,Rommel Rodríguez
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| Data: |
2005-07-01
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| Ano: |
2005
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| Palavras-chave: |
Hep-2 cell line
Drifter technique
Mitotic index
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| Resumo: |
The Hep-2 cell line is derived from laryngeal carcinoma cells and is often utilized as a model in carcinogenesis and mutagenesis tests. To evaluate the proliferative potential of this line, we developed a cytogenetic methodology (drifter technique) to obtain metaphases from cells that loose cellular adhesion when they underwent mitosis in culture. By this procedure, 2000 cells were counted, resulting in a mitotic index (MI) of 22.2%. Although this MI was not statistically different from the one obtained using either a classical cytogenetic method or a cell synchronization technique, the drifter technique has the advantage of not requiring the use of some reagents for the obtention of metaphases and also of diminishing the consumption of maintenance reagents for this cell line.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500005
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132005000500005
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.48 n.4 2005
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| Direitos: |
info:eu-repo/semantics/openAccess
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