| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Viability of bull spermatozoa collected from the epididymis stored at 18-20°C
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| Autores: |
Bertol,Melina Andrea Formighieri
Weiss,Romildo Romualdo
Thomaz-Soccol,Vanete
Kozicki,Luiz Ernandes
Fujita,Aline Silva
Abreu,Renata Azevedo de
Green,Kerriel Thandile
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| Data: |
2013-10-01
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| Ano: |
2013
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| Palavras-chave: |
Bull
Epididymis
Orchiectomy
Sperm
Room temperature
Viability
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| Resumo: |
The aim of this study was to evaluate the viability of bull spermatozoa collected from the cauda epididymis stored at 18-20°C, which were compared with semen collected by electro-ejaculation method and preserved at 5°C. Ten pairs of testes from Tabapuã bulls were removed by orchiectomy and stored for 6 (G6), 12 (G12), 18 (G18), 24 (G24) and 30 (G30) h at room temperature (18-20°C). Seven days before orchiectomy, semen was collected by electro-ejaculation method. The sperm parameters evaluated were: sperm motility, vigor, concentration, morphology and acrosome defects. Sperm motility declined (p<0.05) when spermatozoa were stored for 30 h in the epididymis. The spermatozoa from the epididymis showed lower sperm motility than that of spermatozoa collected via electro-ejaculation. There was a little expressive decrease in sperm vigor and increased in morphological defects with storage time, but the acrosome integrity was not affected. Cold storage (5°C) maintained sperm viable for 15 to 40.8 h. Thus, it was possible to recover viable sperm with 41.25% of motility from the cauda epididymis stored at room temperature of 18-20°C for 30 h. There were differences between the ejaculated and epididymal sperm for the bulls and the conservation at 5°C allowed short-term preservation of the gametes.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500008
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132013000500008
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.56 n.5 2013
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| Direitos: |
info:eu-repo/semantics/openAccess
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