| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR
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| Autores: |
Leão,Evelynne Urzêdo
Spadotti,David Marques de Almeida
Rocha,Kelly Cristina G.
Lima,Élison Fabricio B.
Tavella,Luciana
Turina,Massimo
Krause-Sakate,Renate
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| Data: |
2017-01-01
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| Ano: |
2017
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| Palavras-chave: |
Molecular identification
Real-time PCR
Tospovirus vector
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| Resumo: |
ABSTRACT Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to identify Frankliniella schultzei, an important and widespread pest thrips vector of tospoviruses in South America countries. Species-specific primers designed in the mitochondrial cytochrome oxidase I gene (mtCOI) were optimized for detection by conventional PCR and real-time PCR. The primers were tested on immature and adult thrips collected from crops and weeds found in São Paulo State. All samples collected were identified as F. schultzei, indicating the high prevalence of this species as vector of tospoviruses in Brazilian fields.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100454
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/1678-4324-2017160425
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.60 2017
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| Direitos: |
info:eu-repo/semantics/openAccess
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