| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Interference of a commercial catalase preparation in laccase and peroxidase activities
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| Autores: |
Ballaminut,Nara
Yamanaka,Renata
Machado,Katia Maria Gomes
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| Data: |
2009-10-01
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| Ano: |
2009
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| Palavras-chave: |
Peroxidase
Fungi
Basidiomycetes
Hydrogen peroxide
Enzymatic inhibition
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| Resumo: |
The influence of commercial catalase preparations (fungal and bovine origin) on laccase and peroxidase activity assays was evaluated using enzymatic extracts obtained from several basidiomycetes grown under different culture conditions. No hydrogen peroxide was detected in the extracts. Inhibition of laccase activity by 40 to 80% was related to the catalase source. In addition, oxidation of the substrate (ABTS) by fungal catalase in the absence of the enzymatic extract from basidiomycetes was observed. The results demonstrated the need for the evaluation of interference of the commercial catalase preparation when its use was required in the reaction mixture.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500017
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132009000500017
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.52 n.5 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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