| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5
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| Autores: |
Lima,Francisco Esmaile de Sales
Batista,Helena Beatriz Ruthner
Campos,Fabrício Souza
Kunert Filho,Hiran Castagnino
Cibulski,Samuel
Finoketti,Fernando
Arantes,Thalita
Spilki,Fernando
Roehe,Paulo Michel
Rijsewijk,Franciscus Antonius Maria
Franco,Ana Cláudia
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| Data: |
2014-02-01
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| Ano: |
2014
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| Palavras-chave: |
BoHV-5
V mRNA
Transfection
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| Resumo: |
This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100007
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132014000100007
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.57 n.1 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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