| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Production and characterization of amylases from Zea mays malt
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| Autores: |
Biazus,Joana Paula Menezes
Souza,Roberto Rodrigues de
Márquez,Jesus Espinoza
Franco,Telma Teixeira
Santana,José Carlos Curvelo
Tambourgi,Elias Basile
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| Data: |
2009-08-01
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| Ano: |
2009
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| Palavras-chave: |
Maize seed
Malting
Α and β-amylase
Biochemistry characterization
Germination model
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| Resumo: |
In this work the α and β-amylase enzymes were obtained from maize (Zea mays) malt and were biochemistry characterized. A germination study to obtain the maize malt with good amylase activity was made. The maize seeds were germinated in laboratory and the enzymatic activity was measured daily. Activity dependence to germination time were fitted to an exponential model (A=A0eµt), which showed that the behaviour of enzymatic activity in the germination process was similar to the growth of the microorganism. Its model could be applied to describe the mechanism of α-amylase production for each maize varieties and others cereals. Maize malt characterization showed that α and β-amylase had optimal pH between 4-6.5, optimal temperature 50 and 90ºC, and molecular weight of 67.4 and 47.5kDa, respectively. This work contributed with the advances in biotechnology generating of conditions for application of a new and of low price amylases source.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400024
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132009000400024
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.52 n.4 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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