| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol
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| Autores: |
Barreto,George Emílio Sampaio
Oliveira,Elineusa Silva de
Villar,Flávio Augusto Guerreiro Aragão de
Santos,Gleide Souza dos
Lima,Rute Maria Ferreira
Costa,Silvia Lima
Costa,Maria de Fátima Dias
Egito,Eryvaldo Sócrates Tabosa
El-Bachá,Ramon Santos
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| Data: |
2007-09-01
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| Ano: |
2007
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| Palavras-chave: |
Astrocytes
Brain
Cytotoxicity
3-methylcatechol
Peroxidation
Respiration
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| Resumo: |
The aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 µM.min-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH2-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50% of cells after 72 h was 107 mM. The results of the study indicated a direct relationship between cytotoxicity and 3MC oxidation.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500012
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132007000500012
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.50 n.5 2007
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| Direitos: |
info:eu-repo/semantics/openAccess
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