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Provedor de dados:  BABT
País:  Brazil
Título:  Development of a Rapid and Sensitive Method for Detection of African Swine Fever Virus Using Loop-Mediated Isothermal Amplification
Autores:  Wu,Xulong
Xiao,Lu
Wang,Yin
Yang,Zexiao
Yao,Xueping
Peng,Bin
Data:  2016-01-01
Ano:  2016
Palavras-chave:  African swine fever virus
LAMP
K205R gene
Molecular biology
Resumo:  ABSTRACT A loop-mediated isothermal amplification (LAMP) assay was developed for rapid, sensitive and specific detection of African swine fever virus (ASFV). A set of LAMP primers was designed based on the sequence of the ASFV gene K205R. Reaction temperature and time were optimized to 64 oC and 60 min, respectively. LAMP products were detected by agarose gel electrophoresis or visually with the addition of fluorescent dye. The detection limit of the LAMP assay was approximately 6 copies of the target gene per microliter, 100 times more sensitive than conventional PCR. LAMP is a simple and inexpensive molecular assay format for ASFV detection. To date, African swine fever has not been reported in China. LAMP can be used to monitor ASFV spread into China, thereby reducing the threat of ASF.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200400
Editor:  Instituto de Tecnologia do Paraná - Tecpar
Relação:  10.1590/1678-4324-2016160500
Formato:  text/html
Fonte:  Brazilian Archives of Biology and Technology v.59 n.spe 2016
Direitos:  info:eu-repo/semantics/openAccess
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