| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Production of leptospiral LipL32 antigen in Pichia pastoris and its use in an enzyme-linked immunosorbent assay
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| Autores: |
Monte,Leonardo Garcia
Leal,Fernanda Munhoz Dos Anjos
Hartwig,Daiane Drawanz
Vasconcellos,Sílvio Arruda
Brihuega,Bibiana
Dellagostin,Odir Antonio
Hartleben,Cláudia Pinho
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| Data: |
2014-06-01
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| Ano: |
2014
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| Palavras-chave: |
Pichia pastoris
LipL32
ELISA
Leptospirosis
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| Resumo: |
The production of recombinant LipL32 protein using Escherichia coli has been used extensively for the development of vaccines and diagnostic tests for leptospirosis. However, E. coli has demonstrated limitations, including low yield and lack of post-translational modifications. In this study, rLipL32 was produced in eukaryotic expression system (Pichia pastoris) and evaluated the antigen by enzyme-linked immunosorbent assay (ELISA). The yield obtained from the culture supernatant reached 270 mg/L and ELISA showed an accuracy of 95.34%. In summary, the production of rLipL32 using P. pastoris did not impair the antigenic characteristics of this antigen and ensured its use for detecting the leptospiral antibodies in swine sera.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300007
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132014005000001
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.57 n.3 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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