| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples
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| Autores: |
Zanella,Janice
Costa,Sergio Olavo Pinto da
Zacaria,Jucimar
Echeverrigaray,Sergio
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| Data: |
2012-02-01
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| Ano: |
2012
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| Palavras-chave: |
Acanthamoeba
Monocystic culture
Micromanipulation
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| Resumo: |
Acanthamoeba are abundant in a wide range of environments, and some species are responsible for cutaneous infections, keratitis, and granulomatous amoebic encephalitis (GAE). The conventional detection and isolation of amoeba from clinical and environmental samples involves sampling and culture on non-nutrient Ágar medium. Although efficient, this system requires several transfers in order to eliminate contaminants, and is not appropriate for the isolation of individual amoeba from samples with a biodiverse community. In this study we propose an alternative method for the isolation of monocystic clones of Acanthamoeba. The propose method involves sampling, enrichment, encystment induction, and direct cysts micromanipulation and culture on Ágar plates.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100001
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132012000100001
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.55 n.1 2012
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| Direitos: |
info:eu-repo/semantics/openAccess
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