| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Influence of starch and casein administered postruminally on small intestinal sodium-glucose cotransport activity and expression
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| Autores: |
Guimaraes,Kátia Cylene
Hazelton,Sarah Rodrigues
Matthews,James Clyde
Swanson,Kendall Carl
Harmon,David Lee
Branco,Antonio Ferriani
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| Data: |
2007-11-01
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| Ano: |
2007
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| Palavras-chave: |
Cattle
Glucose
Maltase
Sodium-dependent glucose co-transporter
Starch
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| Resumo: |
Twenty-four Holstein steer calves (88 ± 3kg) with abomasal catheters were randomly assigned within blocks to one of four treatments. The treatments consisted of four abomasal infusions: water (control), 4 g/kg BW.d-1 of partially hydrolyzed starch (SH), 0.6 g/kg BW.d-1 of casein, and a mix of SH + casein. The small intestine was collected and five equidistan, 1m sites were identified (duodenum, jejunum 1, jejunum 2 jejunum 3 and ileum). Maltase specific activity in mucosal homogenate and brush border membrane vesicles, SGLT1 protein abundance, and sodium-dependent glucose uptake in brush border membrane vesicles did not differ between the calves receiving different abomasal infusion treatments. However, maltase specific activity in homogenates and brush border membrane vesicles increased four-fold from the duodenum to the first jejunal site before declining steadily towards the ileum (p=0.0145 p=0.0020, respectively). The SGLT1 abundance differed by intestinal sampling site (p=0.0162). These data indicated that cattle might not have the ability to alter the capacity for glucose uptake when challenged with different substrates and that the regulation of SGLT1 expression could differ between bovine and other species.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700007
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132007000700007
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.50 n.6 2007
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| Direitos: |
info:eu-repo/semantics/openAccess
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